High-Performance Liquid Chromatography (HPLC) is one of the most widely used analytical techniques, yet the theory underpinning liquid-phase separations is often poorly understood. This masterclass introduces participants to the important chemical factors that influence HPLC separations (including polarity, acid-base theory, and hydrogen bonding), chromatographic theory, troubleshooting, and method development/validation.
Each day ends with an interactive workshop session.
Learning Objectives
By the end of this three-day intensive course, you will:
Each day ends with an interactive workshop session.
Learning Objectives
By the end of this three-day intensive course, you will:
- Understand HPLC theory
- Be able to adopt a logical approach to HPLC method development
- Know how to approach HPLC troubleshooting
- Understand the causes of poor method robustness
- Be able to design a method validation study that will satisfy regulatory requirements
- Understand the link between method validation and measurement uncertainty
- Be able to use a systematic problem-solving approach to HPLC troubleshooting
